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Strep tactin-hrp

Web同时EGFP的C端含有编码8×His标签的序列和编码短肽亲和标签Strep-tag II的序列,因此可以非常便捷地使用镍柱或Strep-Tactin系统进行分离纯化。同时在目的蛋白和EGFP之间含有PreScission酶切位点,便于后续酶切去除标签。 和EGFP融合表达,便于观察表达水平。 WebStrep-tag® and Strep-Tactin® Purification and Detection Reagents; DNA Purification; Streptavidin; Immunoprecipiation Using Selector Resins; ... Strep-Tactin HRP Conjugate: 2-1502-001: 0.5 ml: Strep-Tactin® coated microplates: 2-1501-001: 10x96 Well Plate: Strep-Tactin® coated microplates: 2-1501-001:

Strep-Tactin® HRP conjugate 2-1502-001

WebStrep-Tactin® HRP Conjugate (Strep-Tactin® labeled with horseradish peroxidase) is used for fast and direct detection of Strep-tag®II fusion proteins (N- or C-terminal or internal … WebThe affinity system based on the artificial peptide ligand Strep-tag® II and engineered tetrameric streptavidin, known as Strep-Tactin®, offers attractive applications for the study of... react native breakpoint debugging https://5amuel.com

StrepMAB-Classic (Strep-tag® II specific monoclonal antibody), HRP …

WebOverview. The Strep•Tag II Antibody HRP Conjugate* is a peroxidase-conjugated Strep•Tag II Monoclonal Antibody* that is useful as a single detection reagent thus eliminating cross … WebThe small Strep-tag II (WSHPQFEK) can be used to purify active, native conformation proteins to high purity on Strep-Tactin resins.In addition, it can be used in combination with the His tag in a standardized two-step purification procedure. Strep-Tactin is available as Superflow chromatography resin for larger scale purification (up to 9 mg/ml resin). WebRecombinant Strep tag fused human Her2 (23-652 aa) was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti- Strep-Tag (clone BL26882) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection. react native browser

Strep-Tactin®XT 4Flow® Starter Kit 2-5998-000 - IBA Lifesciences

Category:Protein detection - IBA Lifesciences

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Strep tactin-hrp

Localization of Ubiquinone-8 in the Na+-pumping NADH:Quinone ...

WebThermo Scientific Pierce High Sensitivity Streptavidin-HRP, 0.5 mL is an exclusive peroxidase-conjugated streptavidin biotin-binding protein that provides signal amplification like poly-HRP and exceptional storage stability. This specially-manufactured variety of HRP-conjugated streptavidin protein meets the demands of today's scientists for ... WebIBA Lifesciences strep tactin conjugated to horseradish peroxidase 1 Price from $9.99 to $1999.99 strep tactin conjugated to horseradish peroxidase - by Bioz Stars , 2024-03 94 / 100 stars Images strep tactin conjugated to horseradish peroxidase ( IBA Lifesciences ) IBA Lifesciences is a verified supplier IBA Lifesciences manufactures this product

Strep tactin-hrp

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WebThe streptavidin variant Strep-Tactin is available as conjugate with horseradish peroxidase (HRP) for fast and directed detection of N-terminally, C-terminally, or internally tagged Strep-tagII or Twin-Strep-tag fusion proteins transferred to a blotting membrane or … Web本申请涉及用于检测可疑含有靶物的样品中的靶物的方法。该方法包括将样品和附着于磁性颗粒上的第一结合分子与附着于固体支持物上的第二结合分子接触。所述第一结合分子能够结合第二结合分子,靶物能够干扰这种结合。施加磁力使得磁性颗粒与固体支持物紧密接近。

WebOct 12, 2015 · Strep-Tactin, an engineered form of streptavidin, binds avidly to the genetically encoded peptide Strep-tag II in a manner comparable to streptavidin binding to … WebOverview. The Strep•Tag II Antibody HRP Conjugate* is a peroxidase-conjugated Strep•Tag II Monoclonal Antibody* that is useful as a single detection reagent thus eliminating cross …

WebThe streptavidin variant Strep-Tactin is available as conjugate with horseradish peroxidase (HRP) for fast and directed detection of N-terminally, C-terminally, or internally tagged … WebApplication. Strep-Tag II Antibody HRP Conjugate produces a strong signal at a 1:4000 dilution for dot blots and western blots. Packaging. 75 μL in Plastic ampoule. Warning. …

Web125 µl, StrepTactin and horseradish peroxidase (HRP) conjugate used for detection of Precision Plus Protein unstained standards, 50 applications Precision Protein StrepTactin …

WebApr 11, 2024 · Therefore, we used the Strep-tag/Strep-Tactin system in this study to isolate proteins bound to AFP. ... reacted with the anti-LAMP-1 antibody and was washed 3 times with TBST buffer and then reacted with a 5000-fold diluted HRP-conjugated anti-rabbit IgG antibody (#W4011, Promega, Alexandria, NSW, Australia). After washing three times with ... react native build debug apkWebStrep-tag® and Strep-Tactin® Purification and Detection Reagents Human Cells & Tissue Fetal Bovine Serum IBA Lifesciences Products Products Products Overview Antibodies/Markers Antibodies/Markers Overview Primary Antibodies Secondary Antibodies Antibody Supporting Products Human Cells & Tissue Human Cells & Tissue Overview how to start saving to buy a houseWebThe Strep-tag II is a synthetic peptide consisting of eight amino acids ( Trp - Ser - His - Pro - Gln - Phe - Glu - Lys ). This peptide sequence exhibits intrinsic affinity towards Strep … how to start sayaka class trialreact native build ipaWebMar 30, 2016 · For detection of the constructs, Strep-Tactin®-HRP conjugate was used (IBA, Göttingen, Germany, Cat. no.: 2-1502-001). Samples were run in triplicates on a 10% SDS-PAGE gel and afterwards blotted for 1 h onto a nitrocellulose membrane. The membrane was blocked with 1 x PBS, 0.5% Tween 20 supplemented with 3% BSA for 1 h. ... react native build app bundleWebNov 18, 2011 · The sample was transferred to a quartz cuvette and illuminated with a 6-watt 365 nm UV lamp (Bachofer-Laboratoriumsgeräte) at 4 °C for the indicated times. Biotinylated proteins were detected by Western blot analysis using a Strep-Tactin horseradish peroxidase conjugate according to the procedures described by the supplier … how to start saving up for an apartmentWebWestern Blot with Strep-Tactin® or StrepMAB conjugates - Protocol 3 3.3.5 Directly add 2.5 µl Strep-Tactin®HRP (1:4000). Incubate 60 min at room temperature, gently shaking 3.3.6 … how to start saving up money