Web1 Oct 2004 · serine recombinases such as Hin generate double-strand breaks in each recombining partner. Here, site-directed protein crosslinking is used to elucidate the configuration of protein subunits and DNA within the Hin synaptic complex and to follow the movement of protein subunits during DNA strand exchange. Our results show that the … Web11 Nov 2008 · Background: Inhibition of tyrosine kinases, including platelet-derived growth factor receptor, can reduce pulmonary arterial pressure in experimental and clinical …

Resolving integral questions in site-specific recombination

Web16 Jan 2004 · Integrases may be grouped into two major families, the tyrosine recombinases and the serine recombinases, based on their mode of catalysis. Tyrosine family integrases, such as λ integrase, utilize a catalytic tyrosine to mediate strand cleavage, tend to recognize longer attP sequences, and require other proteins encoded by the phage … WebSerine and tyrosine recombinases are generally encountered only in prokaryotes and their viruses, namely, eubacteria, archaea, and temperate bacteriophage. Flp and related … top in 36526 car insurance

Site-specific recombination - Wikipedia

WebSSRs belong to two main families, the serine and tyrosine recombinases. The former mediate unidirectional (irreversible) recombination events that produce stable genetic … WebThey use a serine residue rather than a tyrosine to break the DNA strands, and are structurally and mechanistically unrelated to the tyrosine recombinases (Grindley et al., 2006). The newly identified phage integrases had an N-terminal domain that could be aligned with the known serine recombinases, but they were much larger proteins, typically … WebThis shows that even in this case serine is by far preferred over tyrosine by CK2. The superiority of the seryl peptide is due to both much lower K m and much higher V max . … pinch blepharoplasty