Cfse antibody
WebAntibody Type Monoclonal Host Species Rat Immunogen TLR9-GFP transfected cell line Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) Preparation The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. Concentration WebCFSE is a versatile tool for the fluorescent intracellular labeling of live cells. Labeled cells can be assayed using flow cytometry and fluorescent microscopy. The dye is long lasting …
Cfse antibody
Did you know?
WebOct 26, 2010 · Antibody "presented" to T cells in this manner clearly generates a more effective proliferative signal than soluble anti-CD3 alone or anti-CD3 immobilized on a plastic surface . ... (CFSE) was purchased from Molecular Probes (Eugene, OR) and recombinant human IL-2 was purchased from PeproTech (Rocky Hill NJ). WebJan 19, 2024 · Using NSCLC cell lines, we demonstrated that ILT4 was upregulated by both tyrosine kinase mutation-induced and epidermal growth factor (EGF)-dependent EGFR activation and subsequent AKT/ERK1/2 phosphorylation. Overexpressed ILT4 in EGFR-activated tumor cells induced TAM recruitment and M2-like polarization, which impaired …
WebMar 29, 2016 · 28th Mar, 2016. Andre Luiz Zorzetto-Fernandes. BioLegend. I don't think it's possible to uso CFSE proliferation assay for FITC sorted cells because both FITC and CFSE are excited by the blue laser ... WebOct 12, 2010 · Carboxyfluorescein succinimidyl ester (CFSE) is an effective and popular means to monitor lymphocyte division. CFSE covalently labels long-lived intracellular …
WebT-cell proliferation assay was performed using CFSE-loaded PBMCs in the presence of neutralizing anti-HLA-G antibody (αHLA-G Ab) or isotype control antibody (IgG2a). WebA membrane-bound vital dye, CFSE (carboxyfluorescein diacetate, succinimidyl ester) is successfully diluted with daughter cells after replication, which is useful to assess T cell proliferation in response to specific antigens.
WebApr 4, 2013 · CFSE-high cells were pulsed with 1 μM OVA class I-restricted peptide (SIINFEKL) to make them targets for OT-I cytotoxic T-cell function. CFSE-high (peptide-pulsed) and -low (unpulsed) cells were mixed in a 1:1 ratio and transferred i.v. Draining lymph nodes were analyzed 16 h later to determine the percentage of CFSE-high versus …
WebThe Invitrogen CellTrace CFSE kit is used to monitor distinct generations of proliferating cells by dye dilution. Live cells are covalently labeled with a very bright, stable dye. Every … define bleachedWebWhich is best method for labeling the exosome (PKH, CFSE, or other antibody tagging)? When I labeled the exosome using CFSE, the green fluorescence dye disappeared … define bleachingWebPreparation of CFSE dye solution in DMSO 1. Prepare a 10 mM stock solution of CFSE dye by adding 90 μL DMSO to one vial of CFSE. Mix thoroughly by vortexing. 2. Prepare single-use aliquots of the CFSE stock solution (user-defined volume) using appropriately labeled microcentrifuge tubes or 2-mL cryovials. 3. define bleaching agentWebCFSE (0.5 μM) and CD8+ cells enriched by magnetic separation. On day 3, the percentage of proliferating OT-I T cells was quantified based on the percentage of cells that had diluted the CFSE dye (CFSE-low). The percentage of proliferating OT-I T cells increased following anti-CD47 B6H12-mediated phagocy-tosis of cancer by macrophages (Fig. 2A). fee for rti in indiaWebJan 27, 2016 · CFSE (carboxyfluorescein succinimidyl ester) was used as a dye to specifically stain and thereby label the target cell population, allowing living and … fee for schedule medicareWebJul 2, 2013 · Antigen presentation is the process by which innate immune cells such as macrophages and dendritic cells (antigen-presenting cells, APC) acquire antigens and present them to T cells to initiate the adaptive immune response. define bleaching soapWebJan 27, 2016 · CFSE (carboxyfluorescein succinimidyl ester) was used as a dye to specifically stain and thereby label the target cell population, allowing living and dead … define bleaching trays